TABLE 4.

Validation of PCR array results by independent Taqman assay (medial nasal processes)

miRNA	GD10.5	GD11.0	GD11.5
203	3.9 ± 0.3	16.3 ± 0.8	15.1 ± 0.5
Let-7i	5.7 ± 1.1	10.2 ± 0.7	20.1 ± 1.0
367	−1.8 ± 0.6	−6.0 ± 0.8	−8.3 ± 2.5
302a	−3.2 ± 0.2	−4.3 ± 0.1	−7.2 ± 0.6
20b	1.0 ± 1.0	−61.4 ± 0.1	−89.9 ± 4.7
199a-3p	5.1 ± 0.1	3.9 ± 0.2	6.5 ± 0.1
200b	1.9 ± 0.3	2.3 ± 0.3	3.0 ± 0.3
292-3p	−2.6 ± 0.4	−3.6 ± 0.4	−4.9 ± 0.5
140	1.6 ± 0.2	1.7 ± 0.2	2.8 ± 0.2
127	−2.4 ± 0.2	−1.8 ± 0.3	−3.1 ± 0.4

Total RNA was purified from the paired medial nasal processes of GD10.0, GD10.5, GD11.0, and GD11.5 mouse fetuses and cDNAs prepared as detailed in the Materials and Methods section. The expression of individual miRNAs was determined by real-time PCR and normalized to the expression of SnoRNA135. The data are presented as fold-change ± S.D. (n=3) relative to the level on GD10.0. Positive values are fold-increase and negative values are fold-decrease.