. 2015 Mar 30;10(3):e0120997. doi: 10.1371/journal.pone.0120997

Table 2. Lower limits of detection of LAMP and PCR assays.

(A) Lower limit detection test of spiked samples with DNA extracted by QIAamp DNA minikit
Sample type	Trophozoites/reaction
	10⁴	10³	10²	10	1
Spiked water
LAMP	+^a	+	+	+	+
PCR	+	+	+	+	+
Spiked CSF
LAMP	+	+	+	+	+
PCR	+	+	+	+	+
(B) Lower limit detection test of spiked samples DNA extracted by heating method
Sample type	Trophozoites/reaction
	10⁴	10³	10²	10	1
Spiked water
LAMP	+	+	+	+	± (2/3)
PCR	+	+	+	+	± (1/3)
Spiked CSF
LAMP	+	+	+	+	+
PCR	+	+	+	+	± (1/3)

^a +, triplicated assay showed all positive; ±, triplicated assay showed both positive and negative (positive number/test number);-, triplicated assay showed all negative.