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. 2015 Mar 30;10(3):e0118573. doi: 10.1371/journal.pone.0118573

Fig 2. qRT-PCR study flow diagram.

Fig 2

Differentially expressed genes identified from the microarray study were profiled using qRT-PCR for validation and construction of a multi-gene disease classifier using an extended set of 33 cases and 33 controls. Set A was used to build the prediction model and then this model was tested on set B, consisting of 19 cases and 19 controls.