Table 1. Mapping QTL for rapeseed oil content in partial NCII mating design.
QTL | Type | Position (marker) | Allelic frequency | Bulked segregation analysis | Association mapping | Similar results with other methods | Similar results in previous studies | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Locus 1 | Locus 2 | χ 2 | P-value | LOD | Effect | r2(%) | EBLASSO | GEMMA | Regression-based | CV | ||||
1 | a | CB10597C | 0.54 | 17.46 | 0.0002 | 3.79 | 0.2619 | 1.19 | √ | √ | √ | Ra2E04~S13M08-1-70[56];e8m17-309~Ra3H09[51] | ||
2 | A | Bo3b | 0.40 | 15.52 | 0.0004 | 3.13 | −0.2680 | 1.10 | √ | √ | √ | |||
3 | a | xy2b | 0.73 | 13.40 | 0.0012 | 11.39 | 0.5597 | 3.99 | √ | √ | √ | |||
4 | a | BnGMS488A | 0.86 | 11.01 | 0.0041 | 9.27 | 0.5831 | 2.77 | √ | √ | √ | √ | ||
5 | a | BRAS078A | 0.05 | 8.82 | 0.0122 | 14.04 | −1.2047 | 4.12 | √ | √ | √ | √ | BRAS078A[50]; e8m17-309 ~ Ra3H09 [51];AgCan20a ~ Bras026 [52] | |
6 | a | Ol10-C01 | 0.84 | 7.72 | 0.0211 | 7.60 | −0.4916 | 2.22 | √ | √ | √ | √ | HMR612a ~ HMR612b [53]; E7M5c ~ sN0240A [50] | |
7 | a | Bo2d | 0.64 | 6.68 | 0.0354 | 4.00 | −0.2852 | 1.28 | √ | |||||
8 | d | CB10597C | 0.54 | 4.43 | 0.0352 | 3.10 | −0.5672 | 1.07 | √ | √ | √ | |||
9 | aa | CN64c×SA63a | 0.26 | 0.32 | 18.42 | 0.0001 | 6.28 | −0.3402 | 1.77 | √ | √ | √ | HMR300c ~ MR133.2 [53] | |
10 | aa | BRAS063a×Na10-C06A | 0.29 | 0.62 | 12.68 | 0.0018 | 2.23 | −0.2095 | 0.68 | * | √ | √ | Bras063a [52]; EM16/EM17c~sN12353b [54]; GIFLP106 [57];Na10-C06[51] | |
11 | aa | Bo3a×CB10288B | 0.61 | 0.43 | 12.43 | 0.0020 | 7.25 | 0.3888 | 2.10 | * | √ | HMR403b~MR229 [53] | ||
12 | aa | BnGMS175A×Ra2-G08A | 0.59 | 0.25 | 12.09 | 0.0024 | 4.88 | −0.3124 | 1.42 | √ | √ | |||
13 | ad | CB10431A×CB10234A | 0.82 | 0.71 | 11.86 | 0.0027 | 3.48 | 0.6691 | 1.22 | IGF9014c~pw179b [56]; CB10431[52];CB10234[52];CB10234~ZAAS763[50] | ||||
14 | aa | 20-1c×Ra3-E05D | 0.34 | 0.17 | 11.49 | 0.0032 | 3.57 | 0.2579 | 1.01 | * | * | ODD20/GB2-238~EM1/PM4-400 [55] | ||
15 | aa | BRAS014B×Na14-H11B | 0.46 | 0.85 | 11.18 | 0.0037 | 4.14 | 0.2708 | 1.10 | √ | √ | IGF9014c~pw179b [56]; PM88/PM34-484~SA7/PM56-466 [55];Bras115~W09.CD1 [52]; O|10F04~B0SF1574 [57]; Na14H11[57]; CN32a~E7M5f [50]; | ||
16 | da | CB10139B×BnGMS3B | 0.39 | 0.83 | 10.80 | 0.0045 | 4.20 | 0.7397 | 1.01 | √ | SF19775 [57]; PM88/PM34-378~CB10139-176 [55] | |||
17 | aa | CB10229A×Ra3-E05A | 0.63 | 0.50 | 10.70 | 0.0048 | 6.79 | −0.3869 | 2.15 | √ | snap1200~GIFzip47a [57]; SA89~Na12C01a [51] | |||
18 | aa | CB10036B×CB10343A | 0.25 | 0.75 | 12.29 | 0.0021 | 3.73 | 0.2794 | 1.18 | * | IGF5154c~pX141eE[56];AgCan9~Bras102b[52];EM2/ME14a~Ra2-A01 [54]; CZ1b705119~CZ1b684396 [52] | |||
19 | aa | CN75b×CB10373B | 0.67 | 0.51 | 12.28 | 0.0022 | 5.46 | 0.3431 | 1.61 | √ | HMR438a~HMR310[53] | |||
20 | aa | CN1b×Ra2-E12B | 0.67 | 0.64 | 10.83 | 0.0045 | 6.46 | −0.3794 | 1.87 | * | √ | EM1/BG1-405~SA7/PM63-298 [55] | ||
21 | aa | CN64d×20-1b | 0.58 | 0.46 | 10.65 | 0.0049 | 3.62 | 0.2597 | 1.00 | * | ||||
22 | aa | Ol12-D05B×BnGMS385B | 0.60 | 0.82 | 10.55 | 0.0051 | 3.49 | −0.3293 | 1.07 | * | e18m6-189~CB10028 [51]; E32M48.255~CB10179 [52];niab013~SF25867[57] | |||
23 | aa | MR119d×Na12-A02B | 0.46 | 0.56 | 10.16 | 0.0062 | 5.16 | −0.3079 | 1.44 | √ | √ | √ | √ | ME16/EM17c~sN12353b[54];Na12-A02[51] |
24 | da | E5a×Na14-H11A | 0.46 | 0.74 | 9.19 | 0.0101 | 2.56 | 0.8083 | 0.76 | * | Na14-H11[57];MR216a~MR144[52] | |||
25 | ad | xy2b×CB10343B | 0.40 | 0.50 | 9.19 | 0.0101 | 7.35 | 1.7274 | 1.83 | √ | ||||
26 | aa | MR097×Ol12-F02B | 0.81 | 0.65 | 9.03 | 0.0110 | 5.66 | 0.3232 | 1.65 | √ | E46M64g~Bras089[52];B087P06-1~SA89[51];Ol12-F02A~Mr216b[52];niab028~FITO516c[51] | |||
27 | aa | CB10493C×BnGMS340B | 0.15 | 0.46 | 8.78 | 0.0124 | 3.05 | 0.2502 | 0.96 | √ | * | √ | sORG49a[50] | |
28 | da | CN64d×Ol11-C02A | 0.33 | 0.80 | 8.50 | 0.0143 | 3.05 | −0.6749 | 0.98 | * | * | IGF9014c~E2HM32-320[56] | ||
29 | aa | 32_1a×Na10-C06B | 0.82 | 0.54 | 11.58 | 0.0031 | 4.61 | 0.2997 | 1.37 | * | ODD20/PM16-97~ME2/PM45-384 [55];e4m5-260~CB10632 [51]; ZAAS815b~ZAAS893 [50] | |||
30 | dd | Ra2E12×CB10065B | 0.22 | 0.90 | 9.60 | 0.0019 | 3.00 | 1.1079 | 0.78 | Ra2E12 [56]; FITO131 [51];E38M621~AgCan50 [52];CB10530a~EM9/ME37a [54];CB10065[52];BoGMS1025~SF17359[57] | ||||
31 | aa | BnGMS352B×Ra3-E05C | 0.87 | 0.47 | 9.18 | 0.0102 | 2.63 | −0.2047 | 0.67 | * | e18m6-189~e18m5-374[51];ODD20/GB2-238~EM1/PM4-400[55] | |||
32 | da | CB10045A×Ra3-E05B | 0.22 | 0.15 | 9.01 | 0.0111 | 4.09 | −0.8761 | 1.39 | E2M3/g~EM11/Me23a [54]; E42M50.55~E41M50.206 [52];sORG49a[50] | ||||
33 | dd | Bo2d×BnGMS175A | 0.62 | 0.43 | 9.00 | 0.0027 | 2.13 | −0.5142 | 0.52 | *, * | * | |||
34 | aa | Bo3a×CB10065A | 0.68 | 0.05 | 12.45 | 0.0020 | 2.65 | 0.2238 | 0.77 | * | * | CB10065 [52]; BoGMS1025~BrSF50-42 [57];Na12G11~SN12508 [52]; R04.1840~R06.1360 [52] | ||
35 | aa | BRMS-036c×CB10364B | 0.13 | 0.96 | 11.40 | 0.0033 | 3.35 | −0.2488 | 0.95 | √ | * | BRMS036[50];CB10364**[54];H004I05-1~BnGMS312[51] | ||
36 | ad | CB10493C×BnGMS3B | 0.94 | 0.61 | 9.60 | 0.0082 | 2.41 | 0.6348 | 0.96 | * | IGF2021e~S10M03-1-360[56];e10m22-313~CB10028[51] | |||
37 | da | CN46b×CB10597C | 0.29 | 0.46 | 8.00 | 0.0183 | 2.89 | 0.7535 | 0.81 | Ra2E04~S13M08-1-70[56];e8m17-309~Ra3H09[51] | ||||
38 | aa | BnGMS103B×CB10277B | 0.48 | 0.62 | 8.84 | 0.0120 | 3.51 | −0.2578 | 1.12 | *, * | CB10277[54] | |||
39 | dd | Ra2-E12A×Ol11-C02A | 0.85 | 0.14 | 8.47 | 0.0036 | 3.35 | −0.9321 | 1.09 | RA2E12[56];E38M621~Na12B05[52];IGF9014c~E2HM32-320[56] | ||||
40 | aa | MD21a×Ol12-D05A | 0.69 | 0.22 | 8.11 | 0.0173 | 3.62 | −0.4304 | 0.96 | √ | BrBAC138~GIFLP106[57];E32M48.255~SN11670[52];BRAS031a~E2M3e[50] | |||
41 | aa | BRAS063a×CB10139B | 0.73 | 0.67 | 11.89 | 0.0026 | 4.58 | −0.2801 | 1.20 | √ | * | √ | ||
42 | aa | CN64d×CN59a | 0.08 | 0.22 | 8.66 | 0.0132 | 2.55 | −0.2134 | 0.68 | |||||
43 | aa | xy2a×20-1c | 0.25 | 0.86 | 12.32 | 0.0021 | 2.30 | 0.2178 | 0.71 | * | ||||
44 | aa | CN63b×Na12-A02C | 0.27 | 0.68 | 8.47 | 0.0145 | 2.68 | −0.2385 | 0.76 | * | Na12-A02[51] | |||
45 | da | 20-1b×CB10026C | 0.65 | 0.80 | 12.22 | 0.0022 | 2.15 | 0.5195 | 0.74 | CN32a~E7M5f[50];I20.760~D20.760[52];sN3761b~sR6293b[56] |
a: additive; d: dominant; aa: additive-by-additive; ad: additive-by-dominant; da: dominant-by-additive; dd: dominant-by-dominant; r2: the proportion of total phenotypic variance explained by a single QTL.
EBLASSO: Fast empirical Bayesian LASSO [11];GEMMA: genome-wide efficient mixed-model association study [48]; Regression-based: Regression-based association study [49]; CV: cross validation;
√: same QTL was detected by other methods;
*: locus linked to the detected locus was identified by other methods.