Table I.

Effect of DMBA and/or TQ, BCS and 5-fluorouracil on the measured biochemical parameters in experimental animals (mean ± SEM)

Assays	Treatment
	Control	DMBA	DMBA + TQL	DMBA + TQM	DMBA + TQH	DMBA + BCSL	DMBA + BCSM	DMBA + BCSH	DMBA + FU
MDA	7.1 ±0.2b	15.3 ±0.32a	13.4 ±0.22a	12.7 ±0.34a	9.2 ±0.21b	15.1 ±0.12a	13.8 ±0.14a	10.1 ±0.11b	8.9 ±0.32b
LDH	162.2 ±4.1d	983.6 ± 12.1a	813.6 ± 10.1a	616.7 ± 11.8b	399.1 ± 10.4c	914.3 ± 11.1a	766.1 ± 10.1b	623.2 ± 14.2b	345.6 ± 11.8c
ALP	141.2 ± 2.6c	365.5 ± 14.4a	305.3 ± 11.2a	218.1 ± 9.7b	179.2 ± 8.1c	325.4 ± 12.6a	276.6 ± 8.6ab	219.6 ± 10.1b	168.6 ± 9.2c
AST	24.5 ±0.67c	64.4 ±5.7a	55.5 ±4.8a	39.8 ±3.8ab	29.4 ±3.2b	58.2 ±3.7a	46.3 ±4.4a	38.9 ±4.6a	30.7 ±1.1b
ALT	33.4 ±0.1b	69.7 ±3.7a	57.9 ±4.6a	44.8 ±2.9ab	39.1 ±1.7b	62.8 ±3.6a	51.2 ±1.9a	42.1 ±2.3ab	39.4 ±2.2b

DMBA – 7,12-dimethylbenz[a]anthracene, TQ – thymoquinone, BCS – black cumin seed, FU – 5-fluorouracil, L – low dose, M – medium dose, H – high dose. Means showing different lettering in a row differed significantly at p < 0.05.

^ap < 0.05 compared to control group

^bp < 0.05 compared to DMBA group

^c,dp < 0.05 compared to DMBA + TQ and BCS groups, respectively. Data were analyzed by ANOVA followed by Scheffe test