Table 1.
Gα-Binding and GAP Activity of Arabidopsis thaliana and Setaria italica RGS proteins.
| GTP Hydrolysis Rate (min−1) |
Dissociation Constant (M) |
|||
|---|---|---|---|---|
| RGA | AtGPA1 | RGA | AtGPA1 | |
| AtRGS1 | 0.15 ± 0.01 | 2.52 ± 0.70 | 5.67 × 10−8 | 1.74 × 10−8 |
| SiRGS1 | 2.90 ± 0.30 | 3.08 ± 0.83 | — | — |
| SiRGS1-S75T | 0.50 ± 0.05 | 2.13 ± 0.24 | No interaction observed | 2.19 × 10−8 |
Note.—GTP hydrolysis rate (kcat) values were obtained from a previously published data (Urano et al. 2012) and single turnover assays (supplementary fig. S2, Supplementary Material online). Dissociation constant (KD) values were obtained from a previous publication (Urano et al. 2012) and an SPR experiment (supplementary fig. S4, Supplementary Material online).