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. 2015 Jan 7;32(4):1039–1055. doi: 10.1093/molbev/msu408

Fig. 4.

Fig. 4.

Cellular localization of PFL, ACS, AS, and IPP in Mastigamoeba balamuthi. (A) Immunofluorescence microscopy. Signals for GCSH, AS, and IPP clearly colocalized with hydrogenosomal MDH (PCC = 0.907 ± 0.023, n = 42; 0.902 ± 0.025, n = 41; and 0.876 ± 0.023, n = 38, respectively). ACS colocalized with MDH. However, some signal was observed in the cytosol (PCC = 0.659 ± 0.047, n = 43). Signal for PFL was consistent with its cytosolic localization (PCC = 0.411 ± 0.063, n = 39). Proteins were visualized using antibodies against human GCSH and M. balamuthi AS, ACS, IPP, PFL, and MDH. Alexa Fluor 488 donkey α-rabbit and 594 donkey α-mouse and α-rat were used as secondary antibodies. (B) Immunoblot analysis of cellular fractions. Cell lysate (Lys) was separated into cytosolic (Cyto) and hydrogenosome-enriched (Hydro) fractions using differential centrifugation and probed with the appropriate antibodies.