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. 2015 Mar 24;48(Pt 2):464–476. doi: 10.1107/S1600576715003593

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© R. N. Wilke et al. 2015

This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

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(a) The CTF-based reconstruction of region (A) of the B. subtilis sample. Three isolated endospores can be seen. (b), (c) Calculated gradients (filtered with pixel Gaussian) in the horizontal and vertical directions of the region of two endospores of (a). The black arrow heads highlight the transition between two different structural regions of the endospore that are attributed to the coat and the inner part. The repetitive structure on the gradient maps is an artefact of the reconstruction in (a). The scale bars denotes 0.5 µm.

Inline graphic — (a) The CTF-based reconstruction of region (A) of the B. subtilis sample. Three isolated endospores can be seen. (b), (c) Calculated gradients (filtered with pixel Gaussian) in the horizontal and vertical directions of the region of two endospores of (a). The black arrow heads highlight the transition between two different structural regions of the endospore that are attributed to the coat and the inner part. The repetitive structure on the gradient maps is an artefact of the reconstruction in (a). The scale bars denotes 0.5 µm.