. Author manuscript; available in PMC: 2015 Mar 31.

Published in final edited form as: J Biol Methods. 2015 Mar 11;2(1):e14. doi: 10.14440/jbm.2015.39

Table 1.

saRNA design rules.

Rule name	Criterion
Distance from TSS	−100 - −1000
CpG islands	avoid
Target size	19 nt
Target GC content	40-60%
Consecutive nucleotides	<= 5
Simple repeats (di or tri-nucleotide)	avoid
CpGs in target	<= 1
1^st and 2^nd nucleotides	preferably G or C
18th nucleotide	A or T
19th nucleotide	A
20-23th nucleotides	preferably A or T