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. Author manuscript; available in PMC: 2016 Mar 31.
Published in final edited form as: J Am Coll Cardiol. 2015 Mar 31;65(12):1199–1214. doi: 10.1016/j.jacc.2015.01.031

FIGURE 5. Pharmacological Phosphorylation of Cofilin-2 Induces Formation of Stress-like Fibers in Cardiomyocytes.

FIGURE 5

FIGURE 5

(A) Phosphorylation of cofilin-2 is increased following stimulation of RhoA (grey bars) (*p < 0.05) and reduced by ROCK inhibition (black bars) compared to control vehicle (white bars); values are mean ± SD; number of samples indicated in the bars. MW= molecular weight; kDa=kilo-Dalton. (B) RhoA stimulation and ROCK inhibition respectively stimulate and protect from “stress-like” fibers formation in adult mice cardiomyocytes compared to controls (26). Stress-like fibers are indicated by the dotted lines. Phalloidin staining of F-actin, green; phospho-cofilin, red; nuclei stained with DAPI, blue. DAPI = 4′,6-diamidino-2-phenylindole; RhoA = Ras-homolog gene family member A; ROCK = Rho-associated-protein-kinase; other abbreviations as in Figure 2.