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. Author manuscript; available in PMC: 2016 Mar 31.
Published in final edited form as: J Am Coll Cardiol. 2015 Mar 31;65(12):1199–1214. doi: 10.1016/j.jacc.2015.01.031

FIGURE 6. Genetic Overexpression of Phosphomimetic Ser3-Cofilin-2 Induces Formation of Stress-like Fibers in Cardiomyocytes.

FIGURE 6

(A) WT cofilin-1, (B) Ad.S3A (constitutively active), or (C) Ad.S3E (phosphomimetic) infected neonatal cardiomyocytes. Adenoviral expression of the phosphomimetic cofilin-1 increases the formation of “stress-like” fibers (arrowheads). Red-fluorescence-protein reporter gene (RFP) indicates cardiomyocytes infected with Ad.WT, Ad.S3A or Ad.S3E, red; α-Sarcomeric Actin, green; phalloidin staining of F-actin, teal; nuclei stained with DAPI, blue. Other abbreviations as in Figure 2.