Figure 5. In-resin single molecule super-resolution imaging of histones.

(a) Conventional wide-field fluorescence image of H2B-GFP in a HEK293T cell. Blue dashed lines indicate the plasma membrane (PM). (b) Corresponding SMLM image showing a very detailed distribution of H2B-GFP molecules in the nucleus of the cell. (c) Magnified image of region marked with green rectangle in (b). (d) Distribution of detected single molecules corresponding to image (c). Their positions are marked by crosses in a contour map of the local molecule densities. Average single molecule localization accuracy was ~15 nm, local densities of detected molecules reach up to ~40,000 per μm² (~2,000 molecule positions per diffraction limited volume). Scale bars in (a) and (b) are 1 μm, in (c) and (d) 20 nm. (e) Color code for local molecule densities and corresponding Nyquist limited resolution.