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| Function | Volume/time (μL/min) |
Position |
|---|---|---|
| Draw | 2.5 | Borate vial 1 |
| Draw | 0.5 | Sample vial |
| Mix | 3.0 | Washport 5 × |
| Wait | 0.2 | ─ |
| Draw | 0.5 | OPA vial 2 |
| Mix | 3.5 | Washport 6 × |
| Draw | 0.04 | FMOC vial 3 |
| Mix | 3.9 | Washport 10 × |
| Draw | 32 | Diluent vial 4 |
| Mix | 20 | Seat 8 × |
| Inject | ─ | ─ |
| Wait | 0.1 | ─ |
| Valve bypass | ─ | ─ |
*HPLC, high-performance liquid chromatography; OPA, O-phthaldialdehyde; FMOC, fluorenyl methoxy carbonyl chloride.
