Figure 7. Effects of Nrg1 reactivation on cardiac function.
(A) Doppler measures of ventricular filling obtained at the AV valve in cmlc2:CreER; β-act2:BSNrg1 and β-act2:BSNrg1 animals (n = 9 and 7). Data are represented as mean ± SEM. *p < 0.05, Student's t-test, two-tailed. (B). Representative PW Doppler at the AV valve in cmlc2:CreER; β-act2:BSNrg1 and β-act2:BSNrg1 animals. Arrows indicate E waves. (C) Doppler measures of cardiac output and stroke volume using the velocity time integral (VTI) obtained at the outflow tract (OFT) in cmlc2:CreER; β-act2:BSNrg1 and β-act2:BSNrg1 animals (n = 9, 7). Data are represented as mean ± SEM. *p < 0.05, Student's t-test, two-tailed. (D) Representative PW Doppler at the OFT in cmlc2:CreER; β-act2:BSNrg1 and β-act2:BSNrg1 animals. (E) Quantification of graded swimming performance of animals at varying times of nrg1 overexpression plotted as box and whisker plots. Two-way ANOVA was performed looking at the effect of Nrg1 overexpression (p < 0.05), age (p = 0.38), and the interaction of Nrg1 overexpression and age (p < 0.05).