Table 1.
Band No.a | Description | Accession number | MW (Da)b | pIc | Peptides Matches | Coverage (%) | Species |
---|---|---|---|---|---|---|---|
1 | Hypothetical TonB-dependent receptor | gi|24372887 | 110.093 | 4.4 | 4 | 10 | Shewanella oneidensis |
Hypothetical TonB-dependent receptor | gi|24374013 | 97.761 | 4.5 | 9 | 23 | Shewanella oneidensis | |
Hypothetical TonB-dependent receptor | gi|24373387 | 101.775 | 4.6 | 7 | 17 | Shewanella oneidensis | |
Probable RND efflux transporter | gi|15599571 | 110.533 | 5.2 | 9 | 23 | Pseudomonas aeruginosa | |
Peptidase, M16 family | gi|24375509 | 104.287 | 5.3 | 9 | 15 | Shewanella oneidensis | |
Organic solvent tolerance protein OstA | gi|15595792 | 104.273 | 5.4 | 6 | 15 | Pseudomonas aeruginosa | |
AcrB/AcrD/AcrF family protein | gi|24373487 | 113.613 | 5.6 | 9 | 23 | Shewanella oneidensis | |
Polysaccharide biosynthesis protein | gi|24374706 | 100.376 | 5.7 | 7 | 17 | Shewanella oneidensis | |
Probable glycosyl hydrolase | gi|15597358 | 103.694 | 5.9 | 9 | 23 | Pseudomonas aeruginosa | |
Probable ferredoxin | gi|15599966 | 102.625 | 6.2 | 6 | 15 | Pseudomonas aeruginosa | |
IgA1 protease | gi|12597970 | 110.153 | 7.5 | 8 | 20 | Neisseria meningitidis | |
Probable adhesin | gi|15599277 | 100.434 | 7.7 | 10 | 25 | Pseudomonas aeruginosa | |
2 | Flagellin | gi|16901494 | 50.853 | 4.7 | 23 | 67 | Escherichia coli |
FliC | gi|30059880 | 48.107 | 4.8 | 20 | 58 | Escherichia coli | |
Flagellin | gi|38049856 | 52.274 | 5.0 | 15 | 71 | Salmonella enterica | |
3d | Membrane-associated Zn-dependent protease | gi|71738080 | 48.561 | 5.0 | – | – | Pseudomonas syringae |
Possible peptidoglycan-binding LysM | gi|68181658 | 51.641 | 6.8 | – | – | Jannaschia sp. | |
4 | Survival protein surA | gi|24375136 | 48.914 | 5.7 | 6 | 16 | Shewanella oneidensis |
TolB protein | gi|24372890 | 46.280 | 5.8 | 4 | 11 | Shewanella oneidensis | |
PobA protein | gi|6682326 | 44.121 | 5.9 | 9 | 25 | Pseudomonas putida | |
Serine protease, HtrA/DegQ/DegS family | gi|24375430 | 46.551 | 6.0 | 4 | 11 | Shewanella oneidensis | |
Flagellar hook-associated protein FliD | gi|24374747 | 47.914 | 7.7 | 7 | 19 | Shewanella oneidensis | |
Periplasmic sulfate-binding protein | gi|24375099 | 37.109 | 8.5 | 4 | 11 | Shewanella oneidensis | |
RND multidrug efflux membrane protein MexE | gi|15597689 | 45.031 | 8.5 | 8 | 22 | Pseudomonas aeruginosa | |
5 | Putative outer membrane porin | gi|24371910 | 42.311 | 4.5 | 5 | 12 | Shewanella oneidensis |
Maltoporin | gi|16131862 | 49.913 | 4.8 | 9 | 25 | Escherichia coli | |
Putative adhesin/invasin | gi|21427120 | 41.307 | 4.9 | 12 | 30 | Neisseria meningitidis | |
TolB domain protein | gi|24372890 | 46.280 | 5.8 | 5 | 12 | Shewanella oneidensis | |
Cation efflux system protein cusB precursor | gi|2495562 | 44.305 | 5.9 | 13 | 32 | Escherichia coli | |
HlyF | gi|32470076 | 41.022 | 6.8 | 8 | 20 | Escherichia coli | |
Probable porin | gi|15595437 | 46.125 | 7.8 | 7 | 17 | Pseudomonas aeruginosa | |
RND multidrug efflux membrane fusion protein | gi|15597689 | 45.031 | 8.5 | 8 | 20 | Pseudomonas aeruginosa | |
Membrane-bound lytic transglycosylase | gi|24372749 | 39.715 | 8.5 | 5 | 12 | Shewanella oneidensis | |
6d | Flagellin | gi|3386642 | 39.507 | 4.7 | – | – | Pseudomonas aeruginosa |
Flagellin | gi|94771 | 40.041 | 5.4 | – | – | Pseudomonas aeruginosa | |
7d | Flagellin | gi|3098299 | 40.377 | 4.5 | – | – | Pseudomonas fluorescens |
FliC | gi|30692080 | 39.432 | 4.8 | – | – | Pseudomonas aeruginosa | |
8 | Structural outer membrane porin OprF | gi|15596974 | 37.640 | 5.0 | 4 | 12 | Pseudomonas aeruginosa |
Probable hydrolase | gi|15600370 | 25.629 | 5.6 | 6 | 18 | Pseudomonas aeruginosa | |
Probable FKBP-peptidyl-prolyl cis-trans isomerase | gi|12231005 | 25.278 | 8.0 | 4 | 12 | Pseudomonas aeruginosa | |
Periplasmic ABC-type phosphate transport | gi|46320586 | 33.141 | 9.0 | 6 | 22 | Burkholderia cepacia | |
Putative glycosyltransferase | gi|5545325 | 35.778 | 9.3 | 4 | 12 | A. actinomycetemcomitans | |
Chaperone protein fimC | gi|26247791 | 26.158 | 9.5 | 4 | 12 | Escherichia coli | |
Peptidase, M23/M37 family | gi|24371648 | 38.450 | 9.6 | 4 | 12 | Shewanella oneidensis | |
9 | MSHA biogenesis protein MshJ | gi|24350496 | 24.838 | 5.1 | 6 | 42 | Shewanella oneidensis |
Probable outer membrane protein | gi|11351562 | 21.748 | 5.3 | 5 | 35 | Pseudomonas aeruginosa | |
Sodium-type flagellar protein MotX | gi|24350265 | 23.210 | 8.4 | 6 | 42 | Shewanella oneidensis | |
Outer membrane lipoprotein (lipocalin) | gi|15804743 | 19.852 | 8.9 | 4 | 28 | Escherichia coli | |
Intimin | gi|1703488 | 29.751 | 9.3 | 4 | 28 | Escherichia coli | |
Pertactin | gi|49243344 | 28.759 | 9.7 | 5 | 35 | Bordetella bronchiseptica |
Band numbers are as indicated in Fig. 5B.
Theoretical molecular mass of the proteins.
Theoretical charge of the proteins.
Peptide sequences obtained using N-terminal as described in Experimental procedures.