Table 1.

Proteins identified by 1D SDS-PAGE from P. antarctica OMV-u.

Band No.^a	Description	Accession number	MW (Da)^b	pI^c	Peptides Matches	Coverage (%)	Species
1	Hypothetical TonB-dependent receptor	gi\|24372887	110.093	4.4	4	10	Shewanella oneidensis
	Hypothetical TonB-dependent receptor	gi\|24374013	97.761	4.5	9	23	Shewanella oneidensis
	Hypothetical TonB-dependent receptor	gi\|24373387	101.775	4.6	7	17	Shewanella oneidensis
	Probable RND efflux transporter	gi\|15599571	110.533	5.2	9	23	Pseudomonas aeruginosa
	Peptidase, M16 family	gi\|24375509	104.287	5.3	9	15	Shewanella oneidensis
	Organic solvent tolerance protein OstA	gi\|15595792	104.273	5.4	6	15	Pseudomonas aeruginosa
	AcrB/AcrD/AcrF family protein	gi\|24373487	113.613	5.6	9	23	Shewanella oneidensis
	Polysaccharide biosynthesis protein	gi\|24374706	100.376	5.7	7	17	Shewanella oneidensis
	Probable glycosyl hydrolase	gi\|15597358	103.694	5.9	9	23	Pseudomonas aeruginosa
	Probable ferredoxin	gi\|15599966	102.625	6.2	6	15	Pseudomonas aeruginosa
	IgA1 protease	gi\|12597970	110.153	7.5	8	20	Neisseria meningitidis
	Probable adhesin	gi\|15599277	100.434	7.7	10	25	Pseudomonas aeruginosa
2	Flagellin	gi\|16901494	50.853	4.7	23	67	Escherichia coli
	FliC	gi\|30059880	48.107	4.8	20	58	Escherichia coli
	Flagellin	gi\|38049856	52.274	5.0	15	71	Salmonella enterica
3^d	Membrane-associated Zn-dependent protease	gi\|71738080	48.561	5.0	–	–	Pseudomonas syringae
	Possible peptidoglycan-binding LysM	gi\|68181658	51.641	6.8	–	–	Jannaschia sp.
4	Survival protein surA	gi\|24375136	48.914	5.7	6	16	Shewanella oneidensis
	TolB protein	gi\|24372890	46.280	5.8	4	11	Shewanella oneidensis
	PobA protein	gi\|6682326	44.121	5.9	9	25	Pseudomonas putida
	Serine protease, HtrA/DegQ/DegS family	gi\|24375430	46.551	6.0	4	11	Shewanella oneidensis
	Flagellar hook-associated protein FliD	gi\|24374747	47.914	7.7	7	19	Shewanella oneidensis
	Periplasmic sulfate-binding protein	gi\|24375099	37.109	8.5	4	11	Shewanella oneidensis
	RND multidrug efflux membrane protein MexE	gi\|15597689	45.031	8.5	8	22	Pseudomonas aeruginosa
5	Putative outer membrane porin	gi\|24371910	42.311	4.5	5	12	Shewanella oneidensis
	Maltoporin	gi\|16131862	49.913	4.8	9	25	Escherichia coli
	Putative adhesin/invasin	gi\|21427120	41.307	4.9	12	30	Neisseria meningitidis
	TolB domain protein	gi\|24372890	46.280	5.8	5	12	Shewanella oneidensis
	Cation efflux system protein cusB precursor	gi\|2495562	44.305	5.9	13	32	Escherichia coli
	HlyF	gi\|32470076	41.022	6.8	8	20	Escherichia coli
	Probable porin	gi\|15595437	46.125	7.8	7	17	Pseudomonas aeruginosa
	RND multidrug efflux membrane fusion protein	gi\|15597689	45.031	8.5	8	20	Pseudomonas aeruginosa
	Membrane-bound lytic transglycosylase	gi\|24372749	39.715	8.5	5	12	Shewanella oneidensis
6^d	Flagellin	gi\|3386642	39.507	4.7	–	–	Pseudomonas aeruginosa
	Flagellin	gi\|94771	40.041	5.4	–	–	Pseudomonas aeruginosa
7^d	Flagellin	gi\|3098299	40.377	4.5	–	–	Pseudomonas fluorescens
	FliC	gi\|30692080	39.432	4.8	–	–	Pseudomonas aeruginosa
8	Structural outer membrane porin OprF	gi\|15596974	37.640	5.0	4	12	Pseudomonas aeruginosa
	Probable hydrolase	gi\|15600370	25.629	5.6	6	18	Pseudomonas aeruginosa
	Probable FKBP-peptidyl-prolyl cis-trans isomerase	gi\|12231005	25.278	8.0	4	12	Pseudomonas aeruginosa
	Periplasmic ABC-type phosphate transport	gi\|46320586	33.141	9.0	6	22	Burkholderia cepacia
	Putative glycosyltransferase	gi\|5545325	35.778	9.3	4	12	A. actinomycetemcomitans
	Chaperone protein fimC	gi\|26247791	26.158	9.5	4	12	Escherichia coli
	Peptidase, M23/M37 family	gi\|24371648	38.450	9.6	4	12	Shewanella oneidensis
9	MSHA biogenesis protein MshJ	gi\|24350496	24.838	5.1	6	42	Shewanella oneidensis
	Probable outer membrane protein	gi\|11351562	21.748	5.3	5	35	Pseudomonas aeruginosa
	Sodium-type flagellar protein MotX	gi\|24350265	23.210	8.4	6	42	Shewanella oneidensis
	Outer membrane lipoprotein (lipocalin)	gi\|15804743	19.852	8.9	4	28	Escherichia coli
	Intimin	gi\|1703488	29.751	9.3	4	28	Escherichia coli
	Pertactin	gi\|49243344	28.759	9.7	5	35	Bordetella bronchiseptica

Band numbers are as indicated in Fig. 5B.

Theoretical molecular mass of the proteins.

Theoretical charge of the proteins.

Peptide sequences obtained using N-terminal as described in Experimental procedures.