Figure 5. D/f plots modulus and slope may reflect FAs and cell spreading.

D/f values of cell spreading (a) with or without 10 μM antibodies directed to integrin α_Vβ₃ on HUVECs. Time progression is indicated with dashed lines. (b) Average of cell spreading progression at different times in the presence or absence of 10 μM antibodies directed to integrin α_Vβ₃. (c) FA per cell after 40 min incubation onto the QCM substrates in the presence or absence of 10 μM antibodies directed to integrin α_Vβ₃. Insets contain representative pictures of HUVEC cells showing vinculin-GFP stained FA in green, DAPI nuclear staining in blue, and TRITC-phalloidin bound to F-actin fibers in red. (d) Representative pictures of cell spreading at 10 and 40 min in the absence (Control) or presence (anti α_Vβ₃) of 10 μM antibodies.