Table 1.
Summary of venom proteins/peptides identified from C. fleckeri venom material. Pressure disrupted nematocysts (PDN); Chemically discharged nematocysts (CDN); a: comparative protein-level results across multiple searches using ProteinPilot; b: The percentage of matching amino acids from identified peptides having confidence greater than or equal to 95%; c: The number of distinct peptides having at least 95% confidence.
| Protein ID Transcriptome Match | Uniprot Accession #(s)/Best Cnidaria BLAST Hit | Conserved Domain | Comparative Protein Level a | %Cov (95%) b | Matched Peptides # (%95) c | |||
|---|---|---|---|---|---|---|---|---|
| PDN | CDN | PDN | CDN | PDN | CDN | |||
| Known Cubozoa Toxins | ||||||||
| T0179 | T1PQV6/Toxin B precursor | – | 44 | 6 | 65.11 | 60.15 | 8 | 63 |
| T0362 | T1PRE3/Toxin A precursor | – | 25 | 7 | 29.7 | 55.3 | 12 | 49 |
| T2746 | A7L035/Toxin CfTX-1 precursor | – | 93 | 40 | 45.9 | 77.9 | 3 | 14 |
| T2621 | A7L036/Toxin CfTX-2 precursor | – | Not found | 68 | Not found | 42.4 | Not found | 10 |
| Metalloproteases | ||||||||
| T0344 | A7S336; Predicted protein; Nematostella vectensis | ShKT domain (IPR003582) | 47 | 27 | 16.92 | 23.69 | 6 | 27 |
| T0690 | A7SNJ4; Metalloendopeptidase; Nematostella vectensis | Peptidase M12A, astacin domain (IPR001506); ShKT domain (IPR003582) | 72 | 61 | 17.49 | 45.73 | 4 | 16 |
| T2821 | A7S5S4; Metalloendopeptidase; Nematostella vectensis | Peptidase M12A, astacin domain (IPR001506) | 177 | 77 | 25 | 47.5 | 2 | 12 |
| T1091 | – | Peptidase M12A, astacin domain (IPR001506) | 207 | 189 | 12.7 | 7.7 | 1 | 2 |
| T2460 | A7T0S0; Metalloendopeptidase; Nematostella vectensis | Peptidase M12A, astacin domain (IPR001506) | 138 | 200 | 25.6 | 41 | 3 | 7 |
| Serine Protease inhibitors | ||||||||
| T0134 | A7SCV8; Predicted protein; Nematostella vectensis | Kazal domain (IPR002350) | Not found | 149 | Not found | 5.34 | Not found | 3 |