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. 2015 Mar 4;7(3):915–938. doi: 10.3390/v7030915

Figure 1.

Figure 1

Effect of gK/pUL20 on the internalization of gD and gH/gL. COS-7 cells were transfected with plasmids expressing gD (a–c) or gH/gL (d–f); together with plasmids expressing GFP plus Cherry (a,d); gM-Cherry plus GFP (b,e); or gK-Cherry plus pUL20-GFP (c,f). At 24 h post-transfection, cells were incubated with gD or gH/gL specific antibodies for 1 h at 37 °C before being fixed, permeabilised and stained with secondary antibody. For clarity, gD and gH/gL signals are shown in black/white in the first column, followed by smaller images of gD and gH/gL signals shown in blue in the second column along with the co-expressed proteins as indicated.