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. 2015 Mar 4;7(3):915–938. doi: 10.3390/v7030915

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Viral protein incorporation in WT, ΔgM, ΔgK and ΔgMΔgK virions. HaCaT cells were infected at 5 PFU/cell and after 24 hpi, (a) cell lysates were prepared and (b) progeny viral particles were isolated from clarified cell culture media by centrifugation through a 33% sucrose cushion. Cell lysates and supernatant-derived virions were separated by SDS-PAGE and analysed using the indicated antibodies. Molecular mass markers (in kDa) are indicated on the right; (c) Using densitometry, packaging levels were calculated by dividing the amount of protein detected in supernatant-derived virions (normalized for gB) by the amount of protein in supernatant-derived virions of WT virus (normalized for gB). Error bars represent standard error of three independent experiments.

Viral protein incorporation in WT, ΔgM, ΔgK and ΔgMΔgK virions. HaCaT cells were infected at 5 PFU/cell and after 24 hpi, (a) cell lysates were prepared and (b) progeny viral particles were isolated from clarified cell culture media by centrifugation through a 33% sucrose cushion. Cell lysates and supernatant-derived virions were separated by SDS-PAGE and analysed using the indicated antibodies. Molecular mass markers (in kDa) are indicated on the right; (c) Using densitometry, packaging levels were calculated by dividing the amount of protein detected in supernatant-derived virions (normalized for gB) by the amount of protein in supernatant-derived virions of WT virus (normalized for gB). Error bars represent standard error of three independent experiments.