Figure 5.

Molecular characterization of exosome preparations uploading Nef^mut/E7 in the presence or not of VSV-G. (A) Western blot analysis of 100 μU of AchE activity of exosomes associating Nef^mut/E7 in the presence or not of VSV-G. Shown are the results obtained upon incubation with either anti-Nef or anti-VSV-G Abs. Arrows indicate the relevant protein products. Molecular markers are given in kDa; (B) FACS analysis of exosomes uploading Nef^mut/E7 in the presence or not of VSV-G. Exosomes were bound to surfactant-free white aldehyde/sulfate latex beads, and then assayed for the contents of GM1 and CD63. Quadrants were set on the basis of the fluorescence of beads alone labeled with CTX-B and anti-CD63 mAb. Results shown in both panels are representative of the assays performed on four different exosome preparations.