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. 2015 Mar 11;7(3):1134–1152. doi: 10.3390/v7031134

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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ICS assays for antigen-specific CD4⁺ and CD8⁺ T cells secreting IFN-γ or IL-4. The spleen from 3 mice of each group were isolated 2 weeks after the last vaccination. The splenocytes were prepared, stimulated, and cultured as described in the Material and Methods. Then the splenocytes were stained with mouse anti-CD4, -CD8, -IFN-γ, and -IL-4 monoclonal antibodies. The CD4⁺ T cells secreting IFN-γ (A) or IL-4 (B); and the CD8⁺ T cells secreting IFN-γ (C) or IL-4 (D) were shown. The data represent the means of subtraction values with SD and were analyzed by one-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001).

ICS assays for antigen-specific CD4⁺ and CD8⁺ T cells secreting IFN-γ or IL-4. The spleen from 3 mice of each group were isolated 2 weeks after the last vaccination. The splenocytes were prepared, stimulated, and cultured as described in the Material and Methods. Then the splenocytes were stained with mouse anti-CD4, -CD8, -IFN-γ, and -IL-4 monoclonal antibodies. The CD4⁺ T cells secreting IFN-γ (A) or IL-4 (B); and the CD8⁺ T cells secreting IFN-γ (C) or IL-4 (D) were shown. The data represent the means of subtraction values with SD and were analyzed by one-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001).