Figure 2.

EV71 titers and CPE in RD cells treatment with distinct siERK (s) or U0126. (A) RD cells, pre-transfected with siERK1, siERK2 or siERK1+2 for 36 h, respectively, or treated with U0126 1 h prior to infection, were infected with EV71 at an MOI of 2. Cells pre-treated with DMSO or siNC were done as parallels. At 24 h p.i., both supernatants and cell lysates were collected and applied for determining the total titers of EV71 by titration; (B) Experiments were performed as described in panel A except CPE was examined every 8 h and images were taken at 24 h p.i.. Each result represents the average of three independent experiments and is shown as the means ± standard deviations (SD). *** p < 0.001, versus corresponding controls by Student’s t-test.