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. 2015 Mar 23;7(3):1429–1453. doi: 10.3390/v7031429

Figure 5.

Figure 5

Cellular FLICE-inhibitory protein (cFLIP; FLICE is FADD-like IL-1beta converting enzyme) and FADD co-delivery improved both cellular immune responses and humoral immune responses for HIV gp140. C57BL/6 mice were immunized with 10 μg plasmid pcDNA3.1-gp140 combined with 10 μg plasmid pJ603-cFLIP or 20 μg plasmid pJ603-FADD at day 0 and day 28. Mice were euthanized 10 days after the last immunization and splenocytes were prepared for cellular immune response assay. Blood was collected during euthanasia to prepare sera for ELISA. Sera were diluted 100-fold for antibody titration. (A) Intracellular cytokine staining; (B) Regulatory T cell assay; (C) Humoral immune responses for IgG2a; (D) Cell proliferation assay for CTLs (top) and Th cells (bottom); Data in A–D are representative of two independent experiments with similar results (n=4 except for control group where n=3). pcDNA3.1 was added to make sure all the vaccinated mice received the same quantity of plasmids.