Table 1.
Modulation of cellular immune responses in mice after DNA vaccination by co-delivering Fas-pathway molecules 1.
| Antigen | Plasmid-Expressed Molecules (Pro- or Anti-Apoptotic) | Ratio | Cellular Immune Responses (CMI) | |
|---|---|---|---|---|
| AL11 Tetramer | CD3CD8 IFNγ | |||
| SIVmac Gag | Fas (pro-) | 1:1 | no change | decrease |
| 1:2,1:3 | decrease | decrease | ||
| shRNA-Fas (anti-) | 1:1,1:4 | no change | decrease | |
| shRNA-FasL (anti-) | 1:1 | no change | decrease | |
| 1:4 | no change | no change | ||
| cFLIP ((anti-) | 1:1 | increase | increase | |
| FADD (pro-) | 1:2 | increase | increase | |
| shRNA-FADD (anti-) | 1:1 | no change | increase | |
| shRNA-caspase 8 (anti-) | 1:1 | increase | increase | |
| HIVBaL gp140 | cFLIP (anti-) | 1:1 | NA | increase |
| FADD (pro-) | 1:2 | NA | increase | |
1 C57 BL/6J mice were immunized with SIVmac Gag plasmid or HIVBaL gp140 plasmid and corresponding pro- or anti-apoptotic molecules. One booster injection was followed 4 weeks later (except for Fas co-delivery with 2 weeks interval). Splenocytes were prepared 10 days after the last immunization. Cellular immune responses were analyzed by AL11 tetramer staining and intracellular cytokine staining for IFNγ. NA means no analysis of CMI. No change in the CMI after plasmid electroporation, with respect to control or empty plasmid is designated no change, whereas increase or decrease in CMI with respect to control plasmids is designated as increase or decrease.