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. 2015 Mar 23;7(3):1454–1473. doi: 10.3390/v7031454

Figure 3.

Figure 3

The recombinant plasmid construction and purification of target proteins. (A) Schematic illustration of SRBSDV P9-1 protein, which has 347 amino acids. Construct names are indicated in the left blocks to represent the P9-1 region contained in the construct. The discontinuous red lines represent the deleted region. The numbers indicate the relative amino acid positions in P9-1. The asterisk (*) on the 138 residues indicates the site of site-directed mutagenesis; (B) expression and purification of the P9-1 protein. The four parts named WT-His-P9-1, TR-ΔC23-His-P9-1, TR-ΔN6-His-P9-1, and MU-138-His-P9-1 represent the desalted proteins. The proteins were separated by 12% SDS–PAGE, with the protein molecular weight standards shown in lane M.