Table 1.
Primer Name | Sequence(5'→3') a |
---|---|
P9-N1 | AAGTTTTTTAAGCCTGGAACTGAC |
P9-C1 | GACATCAGCTGTAAGCCGG |
WT-His-P9-1-N2 | GGAATTCCATATGGCAGACCTAGAGCGTAGAA |
WT-His-P9-1-C2 | CCGCTCGAGTCAAACGTCCAATTTAAGTGAAGAA |
TR-ΔC23-His-P9-1-N3 | GGAATTCCATATGGCAGACCTAGAGCGTAGA |
TR-ΔC23-His-P9-1-C3 | CCGCTCGAGTCAAAAACGACGATATCTTTT |
TR-ΔN6-His-P9-1-N4 | GGAATTCCATATGACGTTTGGATCATATA |
TR-ΔN6-His-P9-1-C4 | CCGCTCGAGTCAAACGTCCAATTTAAGTGAA |
Mu-138-His-P9-1-N5 | CTTTTTGGTCTTTAGTTGTGGATTCGCTTTCAACGAC |
Mu-138-His-P9-1-C5 | GTCGTTGAAAGCGAATCCACAACTAAAGACCAAAAAG |
a Restriction sites are underlined.