Figure 1.

Screening of host cell proteins interacting with VP2 of IBDV by yeast two-hybridization assay. Briefly, the capsid protein VP2 of the IBDV Gt strain was used as the bait in the ProQuest yeast two-hybrid system to screen a cDNA library generated from the chicken bursa of Fabricius cells. The interactions were examined by SD/-Leu/-Trp/-Ura plates and X-gal assay. Ten putative positive colonies were tested for β-galactosidase activity in two additional rounds of selection. The positive (Krev1 and RalGDS-wt) and the negative controls (Krev1 and RalGDS-m2) were performed during each selection round.