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. 2015 Mar 23;7(3):1474–1491. doi: 10.3390/v7031474

Figure 5.

Figure 5

Knockdown of CSGalNAcT2 inhibits IBDV growth. (a) Interference efficiency of siRNAs against exogenous CSGalNAcT2. In DF1 cells, 2 μg pCSGalNAcT2 was co-transfected with 100 pmol negative control siRNA (NC), 1# siRNA, 2# siRNA, or 3# siRNA. Then, CSGalNAcT2 expression was detected using anti-Flag antibody; the results showed that the 2# siRNA and 3# siRNA had obvious effects. (b) The interference efficiency of these siRNAs against endogenous CSGalNAcT2. DF1 cells grown in 12-well plates were transfected with 60 pmol siRNA, and the interference efficiency was determined by real-time RT-PCR at 48 h post-transfection. The results further confirmed the superior interference efficiency of the 2# siRNA and 3# siRNA. Subsequently, cells were infected with the IBDV Gt strain at 48 h post-knockdown of endogenous CSGalNAcT2. IBDV in the cells (c) and supernatants (d) were determined by detecting the content of VP2 or pVP2 protein and viral titre at 24 h post-infection. Knockdown of CSGalNAcT2 inhibited IBDV growth. Values represent the mean ± SEM of three independent experiments. *, p < 0.05, significantly different; **, p < 0.01; ns, no significant difference.