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. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9

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© Decoin et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of hcp1 and hcp2 mutations on flagellin secretion into the extracellular matrix and on motility. A: Protein fractions from the ECM of MFE01, MFE01Δhcp1 and MFE01Δhcp1+hcp1 were analysed by SDS-PAGE and Coomassie staining. Bands with approximate molecular mass ≈ 38 kDa, indicated by the arrows, were observed at the growth temperature of 28°C and were identified as flagellin proteins by MALDI-TOF (n = 3). Molecular weight markers are indicated. B: Swimming assay on LB in 0.3% agar at 28°C. MFE01, MFE01Δhcp2 and MFE01Δhcp1+hcp1 were motile but MFE01Δhcp1 and MFE01Δhcp1+ pPSV35 (empty plasmid used as control) were non motile (n = 3).