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. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9

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© Decoin et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Hcp secretion and killing activity of P. fluorescens MFE01 and derivatives strains. A: Concentrated supernatants of MFE01 and MFE01Δhcp1 cultures were analysed by SDS-PAGE and Coomassie staining. Bands with a molecular mass similar to that of an Hcp protein (≈20 kDa), indicated by the arrow, were observed at a growth temperature of 28°C. MWM: molecular weight markers are indicated. B: Quantitative co-culture assays were performed. Prey cells (MFN1032 carrying pSMC21-gfp) were or were not mixed at ratio of 1:5 with P. fluorescens MFE01, MFE01Δhcp1, MFE01Δhcp 1Δhcp2 and MFE01Δhcp2; after 4 h at 28°C, MFN1032 cfu were counted (n = 4, the error bars represent standard error of the mean). * Indicates a significant difference in MFN1032 cfu (p-value <0.05) relative to the MFN1032/MFE01 assay; ns means no significant difference. ♦ indicates a significant difference in MFN1032 cfu (p-value <0.05) relative to the MFN1032 alone control assay.