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. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9

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© Decoin et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of P. fluorescens MFE01 and its mutants on competitive bacteria. A: Schematic drawing of the assay: P. fluorescens MFN1032 was inoculated into the centre of a ring formed by MFE01 or a mutant on LB medium in 0.3% agar. On this medium MFN1032 and ME01 can swim. B: Swimming of MFN1032 in competition with MFE01 or mutants. Results after incubation at 28°C, 24 h. The images shown are representative of three assays. C: Co-cultures were performed on swarming medium (LB in 0.6% agar) at 28°C, 24h. On this medium MFN1032 can swarm but ME01 cannot. Prey cells (MFN1032) were or were not mixed with P. fluorescens MFE01 or its derivatives at ratio 1:5. The images shown are representative of three assays. D: Quantitative black histograms represent survival of MFN1032 and white squares the MFN1032 swarm diameter (n = 4, error bars represent standard error of the mean). * Indicates a significant difference of the MFN1032 cfu (p-value <0.05) relative to the MFN1032/MFE01 assay. ns: no significant difference.