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. 2015 Mar 26;15:72. doi: 10.1186/s12866-015-0405-9

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© Decoin et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phenotypes of the MFE01∆tssC mutant. A: Swimming assay on LB in 0.3% agar at 28°C during 24 h. MFE01 and MFE01Δhcp2 were motile contrary to MFE01Δhcp1 and MFE01ΔtssC strains (n = 3). B: Representative images of mucoidy of MFE01 and MFE01ΔtssC on LB agar at 28°C, 24 h (n = 3). +: mucoid, −: non mucoid. C: Effects of P. fluorescens MFE01 and its mutants on competitive E.coli on solid medium. Co-cultures were performed on LB medium supplemented with X-Gal (40 μg/ml) at 28°C during 24 h. Prey cells (E.coli DH5αmcr containing pUC19) were or were not mixed with P. fluorescens MFE01 or its derivatives at ratio 1:1. Blue is due to X-Gal degradation by E.colipUC19. The images shown are representative of three assays.