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. 2004 Jul 1;114(1):39–48. doi: 10.1172/JCI20645

Figure 6.

Figure 6

Ovarian mRNA expression of IL-6, IκBα, Socs1, and Socs3 upon systemic CD40 ligation. Whole-ovary mRNA expression levels were determined by real-time RT-PCR. Ovaries were collected from mice on E4 12 hours after treatment with rat IgG or FGK45 (n = 4 mice per group); with FGK45 plus control hamster IgG or FGK45 plus TNF-α–neutralizing mAb’s (n = 5 mice per group); or with rat IgG plus progesterone or FGK45 plus progesterone (n = 4 mice per group). Ovaries were collected on E8 12 hours after injection of rat IgG or FGK45 (n = 3 mice per group). Data represent mean ± SEM. On E4, IL-6, IκBα, Socs1, and Socs3 mRNA levels were increased following FGK45 injection (*P < 0.05; **P < 0.005). These increases were inhibited by TNF-α blockade but not prevented by concurrent progesterone treatment. On E8, FGK45 injection led to upregulated mRNA expression of IL-6 and IκBα, but not Socs1 or Socs3. For ease of graphical representation, transcript levels for IL-6, IκBα, and Socs3 were multiplied by 1,000, 2, and 5, respectively.