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. 2004 Jul 1;114(1):131–140. doi: 10.1172/JCI21136

Figure 6.

Figure 6

The altered endocytic activity of Tf and LDL in cells that expressed R135L/v-arr. Cells transfected with R135L/GFPv-arr (A and B, E and F, I and J, M and N) and WT rhodopsin/GFPv-arr (C and D, G and H, K and L, O and P) were incubated with Alexa 594–conjugated Tf at 37–C for 5 minutes (A and B, C and D), for 2 hours (E and F, G and H), or for 2 hours plus a 30-minute chase (I and J, K and L) before fixation and visualization. Alternatively, cells were treated with DiI-LDL for 2 minutes followed by a 28-minute chase before the fixation (M and N, O and P). Confocal images of GFPv-arr (green) and internalized Tf (red) or LDL (red) are shown. In J and L, the DAPI nuclear labeling demonstrated that the nontransfected cells (in J and L) and WT/GFPv-arr–transfected cells (in L) have no Tf signals, as the Tf was completely expelled from cells. Scale bars: 20 μm.