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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Int J Mass Spectrom. 2015 Feb 1;377:617–624. doi: 10.1016/j.ijms.2014.06.023

Figure 6. Derivatization of human CLIP peptide with histamine enhances fragmentation observed by ETD.

Figure 6

(A) ETD MS/MS spectrum of [M+3H]+3 ions m/z 822.91) from unmodified CLIP. (B) ETD MS/MS spectrum of [M+7H]+7 ions (m/z 433.27) of CLIP derivatized with histamine. Following derivatization, carboxyl groups of five, D and E residues, plus the C-terminus are converted to amides containing a basic imidazole ring. (C) Sequence coverage obtained from (B). Residues labeled by a histamine group are indicated by an asterisk.