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. 2015 Apr 1;128(7):1408–1421. doi: 10.1242/jcs.164467

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Sulf1 does not inhibit the ability of Wnt3a to induce axis duplication. (A–F) Xenopus laevis embryos were microinjected with mRNA encoding Wnt3a (5 pg) and/or Sulf1 (1 ng) into a single ventral blastomere at the four-cell stage. In situ hybridisation for the gene chordin was performed at NF stage 10.5. (A) Uninjected control embryos; (B–D) embryos injected with (B) Sulf1, (C) Wnt3a or (D) Sulf1 and Wnt3a. The areas indicated in the white boxes in C and D are enlarged in E and F, respectively. (G) The data shown in A–F is quantified in G. NS, not significant (Chi squared test), N, number of embryos. (H) Immunoprecipitation (IP) of epitope-tagged proteins expressed in animal caps injected with the mRNAs indicated. The top panel shows protein immunoprecipitated with an antibody against HA (Wnt3a is tagged with HA) and immunoblotted with an antibodies against Myc (LRP6 is tagged with Myc). The bottom two panels are protein lysates prior to immunoprecipitation.