Table 1.
Transcriptome assembly and annotation statistics compared with other Tephritid transcriptomes and the Drosophila melanogaster genome
| Species | B. cucurbitae | B. dorsalis a | C. capitata b | D. melanogaster c |
|---|---|---|---|---|
| Number of read pairs used in assembly (SRA accession number) | ||||
| Egg (SRA: SRS691534) | 43741314 | 12462204 | - | - |
| Larvae (SRA: SRS691533) | 51568835 | 11753084 | - | - |
| Pupae (SRA: SRS691532) | 47093178 | 13291147 | 93256673 | - |
| Adult (SRA: SRS691531) | 46515243 | 47250123 | 96929532 | - |
| Total | 188918570 | 84756558 | 190186205 | - |
| Normalized reads (in silico normalization) | 12792085 | 7796491 | 17217414 | - |
| Unfiltered assembly | ||||
| Number of unigenes (or Drosophila genes) | 50220 | 47216 | 118793 | - |
| N50 longest transcript/unigene | 2191 | 1882 | 1187 | - |
| Sum longest transcript/unigene (Mb) | 49.63 | 40.20 | 81.56 | - |
| Number of transcripts | 76688 | 80345 | 190958 | - |
| N50 transcript length (bp) | 2626 | 2802 | 2686 | - |
| Sum transcript length (Mb) | 100.20 | 109.48 | 236.18 | - |
| Transcripts per unigene | 1.53 | 1.70 | 1.61 | - |
| GC % | 38.10 | 39.11 | 36.21 | - |
| Filtered de novo assembly or current Drosophila release | ||||
| Number of unigenes | 10425 | 10784 | 10741 | 15504 |
| N50 unigene length (longest transcript/unigene) | 3464 | 3043 | 3383 | 2979 |
| Sum longest transcript/unigene (Mb) | 28.12 | 24.46 | 28.34 | 30.53 |
| Number of transcripts | 17654 | 23539 | 21761 | 25205 |
| N50 transcript length (bp) | 3477 | 3460 | 3913 | 3633 |
| Sum transcript length (Mb) | 48.28 | 62.06 | 66.65 | 68.47 |
| Isoforms per unigene | 1.69 | 2.18 | 2.03 | 1.63 |
| GC % | 40.17 | 40.32 | 39.41 | 49.70 |
| N50 protein length (amino acids) | 323 | 301 | 310 | 370 |
| Number of proteins with complete ORF (%) | 12936 (73.2) | 13017 (55.3) | 15740 (72.3) | - |
| Annotation statistics | ||||
| Number of proteins with PFAM domains identified | 13029 | 16612 | 13646 | - |
| Number of proteins with Gene Ontology Terms | 10640 | - | 13648 | - |
| Number of proteins with gene names | 15956 | 17093 | 15841 | - |
| Number of proteins with significant hit to Drosophila proteinsd | 16070 | 20713 | 19245 | - |