Figure 2.

Comparison of the three depolarization methods in HEK cells cotransfected with Ci-VSP, tubbyC, and one of the different channels. (A) Bars representing the maximal response observed by overexpression of KCNQ4, TRPV1, and ChR2. For KCNQ4 we used the tubbyC translocation value from experiments as in graph Figure 1C, for TRPV1 from graph Figure 1E, and for ChR2 the maximal value from the blue light activation in graph Figure 1G. (B) Bars representing t_(1/2) of initiation, meaning the time required to reach 50% of the maximal response, for the three channels, calculated from data summarized in Figures 1C,E,H, respectively. (C) Bars showing t_(1/2) of recovery, the time required for 50% recovery to baseline. Numbers in columns represent the number of cells. ^* Indicates statistically significant difference; ns, not significant.