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. 2015 Mar 31;9:103. doi: 10.3389/fnins.2015.00103

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Copyright © 2015 Prasongchean, Vernay, Asgarian, Jannatul and Ferretti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of survival, proliferation, and doublecortin (Dcx) expression in organotypic cultures of E12 lateral CP. (A) Phase image of CP after 7 days in culture. (B) MTT staining of live CP after 7 days in culture; extensive staining is indicative cell viability. (C) Propidium iodide staining of live CP after 7 days in culture to detect dead cells; only very limited staining is observed. (D,D') CP organotypic culture treated with BrdU for 3 days and double-stained for BrdU and doublecortin (Dcx). Some double-labeled cells are observed. (E) Detection of NF200 and BrdU in an organotypic culture. Scale bars: (A–C) = 200 μm (B and C are at the same magnification); (D,E) = 50 μm.