Table 1.
mitoBKCa biophysical properties in mammals.
| Cell type/Organ/Method | Conductance, [K+], mM Pipette/bath or cis/trans | V½ or Open probability (Po) [Ca2+], μM | Ca2+, EC50 | References |
|---|---|---|---|---|
| Human glioma cell line (LN229) (on-mitoplast) | 295 pS 150 K+/150 K+ | At 8.7 Ca2+, V½ = −33 ±19 mV; At 1 Ca2+, V½ = 41 ± 23 mV | 6.9 μM at −20 mV | Siemen et al., 1999 |
| Guinea-pig ventricular myocytes (on-mitoplast) | 307 pS 150 K+/150 K+ | At 0.512 Ca2+, Po ~ 0.9 from −60 to +60 mV* | N/A | Xu et al., 2002 |
| Rat ventricular myocytes (inside-out mitoplast) | 270 pS 140 K+/140 K+ | At 0.5 Ca2+, Po = 0.0087 at +40 mV | N/A | Ohya et al., 2005 |
| Human glioma cell line (LN229 and LN405) (on-mitoplast) | 276 pS 150 K+/150 K+ | At 200 Ca2+, V½ ~ −42 mV* | N/A | Gu et al., 2007 |
| Rat astrocytes (on-mitoplast) | 295–296 pS 150 K+/150 K+ | At 200 Ca2+, V½ ~ −50 mV* | N/A | Cheng et al., 2008, 2011 |
| Human endothelial cell line (EA.hy926) (on-mitoplast) | 270 pS 150/150 K+ | At 100 Ca2+, V½ ~ +20 mV* | N/A | Bednarczyk et al., 2013b |
| Rat whole brain Lipid bilayers | 265 pS 50 K+/450 K+ | At 0 Ca2+, Po = 0.50 at +70 mV At 300 Ca2+, Po = 0.77 at +70 mV | N/A | Skalska et al., 2009 |
| Rat whole brain Lipid bilayers | 211 pS 200 K+/50 K+ | At “contaminant” Ca2+, Po = 0.9 ± 0.01 at +40 mV, V½ = 11 ± 1 mV | N/A | Fahanik-Babaei et al., 2011a |
| Rat whole brain Lipid bilayers | 565 pS 200 K+/50 K+ | At 100 Ca2+, Po = 0.9 ± 0.05 at −40 to +40 mV. At 0 Ca2+, Po = 0.8 at +20 mV and Po = 0.07 at −40 mV | N/A | Fahanik-Babaei et al., 2011b |
*
Estimated from published figure. On-mitoplast is also called mitoplast-attached configuration of the patch clamp technique. Abbreviations: V½, half activation potential or potential where an open probability of 0.5 is achieved; EC50, concentration of half maximal effect; N/A, not available.