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. 2015 Mar 31;6:95. doi: 10.3389/fphys.2015.00095

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Copyright © 2015 Kumar, Bhardwaj, Kumar and Mazumdar-Leighton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Rubiscolytic activities detected in midgut samples (0.5 μg/μl) from field-collected, fourth instar P. brassicae (n = 36) feeding on leaves of cauliflower (CF-ctrl), T. majus (TP-ctrl), radish (RD-ctrl), cabbage (CB-ctrl), and mustard (MT-ctrl) using native Rubisco isolated from N. tabacum (Nt RBCase) as substrate. (B) Inhibition of rubiscolytic activities detected in the samples after incubation with STI. The extent of inhibition is shown relative to proteolytic activities detected in midgut sample (depicted as 100% in absence of STI). The midgut samples were pre-incubated with STI in 1:1 ratio (v/v) for 10 min at room temperature before adding the protein substrate.