FIGURE 1.

Targeting eGFP to the endogenous PITX3 locus in hPSCs using zinc finger nucleases (ZFN). (A) shows a schematic of the donor plasmid for targeting eGFP to the endogenous PITX3 locus; the strategy employed was based on that reported by Hockemeyer et al. (2009). PGK, human phosphoglycerol kinase promoter; PURO, puromycin resistance gene; loxP, loxP sites; pA, polyadenylation sequence. (B) Correctly targeted colonies (as determined by PCR) were generated with 19% efficiency, and subsequently confirmed by Southern blot analysis (modified from Hartley et al., 2014).