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. 2015 Mar 1;15:13. doi: 10.1186/s12896-015-0127-y

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© Long et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Purification of recombinant tPA. A, SDS-PAGE analysis of tPA purity. Lane 1, protein markers; lane 2, lysate; lane 3, supernatant; lane 4, elution with 200 mM imidazole; lane 5, recombinant tPA purified using ion exchange chromatography; lane 6, PSP; lane 7, PSP enzyme reaction mixtures; lane 8, tPA purification using size-exclusion chromatography. B, Western blotting of the enzyme reaction products (lane 7 of Figure 4A) and the purified fraction (lane 8 of Figure 4A) using anti-tPA antibodies.