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. 2015 Mar 1;15:13. doi: 10.1186/s12896-015-0127-y

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© Long et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Size exclusion gel filtration chromatography of tPA using a Superdex 200 column. tPA (MW ~60.8 kDa) was eluted as a single peak at a volume of 72 ml, which corresponds to a molecular weight of 66.68 kDa on the calibrated column. Standard protein samples (black data points) were used for calibration: albumin bovine V (66.2 kDa), chicken egg albumin (44.2 kDa), chymotrypsinogen A (24.5 kDa), and lysozyme (14.4 kDa).