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. 2015 Mar 25;15:54. doi: 10.1186/s12862-015-0333-1

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© Janssenswillen et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of Sodefrin Precursor Factor. Asterisks show the male breeding glands. (a) PCR-Amplification of SPF precursors in the male cheek and cloaca, female cheek and cloaca, and male skin, gonads and liver, using 11 different primer combinations. The primer combinations are cross-referenced in Additional file 1. For combinations 2–8 and 9–11, comparable results were obtained and only one representative gel is shown. The elongation factor 1-alpha gene (EF1-a) was used as control. (b) Number of recovered SPF transcripts in breeding glands and other tissues of Notophthalmus viridescens.