Table 2.

Correlations between transcription start sites on the forward and reverse strands

Chromosome	z	P	zP	P	TSS forward	TSS reverse	NN
A. Homo sapiens genome
chr1	2.59	0.010	5.93	<0.001	1,226	1,156	230
chr2	3.77	<0.001	4.28	<0.001	817	728	177
chr3	3.01	0.003	4.60	<0.001	643	663	133
chr4	0.28	0.779	2.07	0.038	446	471	103
chr5	4.39	<0.001	4.42	<0.001	570	497	113
chr6	4.34	<0.001	5.22	<0.001	609	617	132
chr7	0.65	0.516	2.97	0.003	562	509	109
chr8	2.76	0.006	4.48	<0.001	397	423	72
chr9	2.97	0.003	3.39	<0.001	443	507	95
chr10	1.97	0.049	4.27	<0.001	471	475	106
chr11	3.52	<0.001	5.02	<0.001	696	656	129
chr12	4.21	<0.001	5.36	<0.001	623	656	147
chr14	4.28	<0.001	4.64	<0.001	382	363	90
chr15	1.94	0.052	5.74	<0.001	338	359	82
chr16	3.73	<0.001	6.30	<0.001	571	422	113
chr17	5.08	<0.001	6.37	<0.001	633	725	150
chr19	4.28	<0.001	7.65	<0.001	823	772	178
chr20	2.57	0.010	4.06	<0.001	320	291	70
chr22	1.77	0.077	4.16	<0.001	268	269	63
chrX	2.24	0.025	1.43	0.153	477	453	96
B. Drosophila melanogaster genome
chr2L	11.98	<0.001	15.68	<0.001	1,393	1,379	454
chr2R	13.72	<0.001	14.73	<0.001	1,578	1,529	481
chr3L	12.57	<0.001	15.59	<0.001	1,528	1,570	473
chr3R	14.19	<0.001	16.61	<0.001	1,762	1,852	583
chrX	12.42	<0.001	14.08	<0.001	1,176	1,198	387

The positions of TSS on the reverse strand were projected to the forward strand. z and z_P are calculated by Equations (23) and (11) and characterize the positional and ordering correlations between TSS, respectively. The 1% significance thresholds for |z| and |z_P| in the case of random correlations correspond to 2.58, while 5% significance thresholds correspond to 1.96. The positive values of z_P indicate that projected TSS on the reverse strand precede TSS on the forward strand. The corresponding P-values were calculated using Gaussian statistics. The data were filtered by the number of pairs of the nearest neighbours (NN) exceeding 50 to ensure the applicability of Gaussian statistics.