Table 1.
Mmp and Timp primers and probe sequences
| Forward primer (5′-3′) | Reverse primer (5′-3′) | Probe (5′-FAM, TAMRA-3′) | |
|---|---|---|---|
| Timp 1 | CATGGAAAGCCTCTGTGGATATG | AAGCTGCAGGCACTGATGTG | CTCATCACGGGCCGCCTAAGGAAC |
| Timp 2 | CCAGAAGAAGAGCCTGAACCA | GTCCATCCAGAGGCACTCATC | ACTCGCTGTCCCATGATCCCTTGC |
| Timp 3 | GGCCTCAATTACCGCTACCA | CTGATAGCCAGGGTACCCAAAA | TGCTACTACTTGCCTTGTTTTGTGACCTCCA |
| Timp 4 | TGCAGAGGGAGAGCCTGAA | GGTACATGGCACTGCATAGCA | CCACCAGAACTGTGGCTGCCAAATC |
| ColA | CGTGGACCAACAGCAGTGAA | GAGTGAGCCCAAGGGAGTGA | TCAACTTGTTCTATGTTACGGCTCATGAACTGG |
| ColB | TGGACCGACAACAATGAGGAT | TGGGAGAGTCCAAGGGAGTG | TCAACTTGTTCTATGTTACGGCTCATGAACTGG |
| MMP-2 | AACTACGATGATGACCGGAAGTG | TGGCATGGCCGAACTCA | TCTGTCCTGACCAAGGATATAGCCTATTCCTCG′ |
| MMP-3 | GGAAATCAGTTCTGGGCTATACGA | TAGAAATGGCAGCATCGATCTTC | AGGTTATCCTAAAAGCATTCACACCCTGGGTCT |
| MMP-7 | GCAGAATACTCACTAATGCCAAACA | CCGAGGTAAGTCTGAAGTATAGGATACA | CCAAAATGGCATTCCAGAATTGTCACCTAC |
| MMP-8 | GATTCAGAAGAAACGTGGACTCAA | CATCAAGGCACCAGGATCAGT | CATGAATTTGGACATTCTTTGGGACTCTCTCAC |
| MMP-9 | CGAACTTCGACACTGACAAGAAGT | GCACGCTGGAATGATCTAAGC | TCTGTCCAGACCAAGGGTACAGCCTGTTC |
| MMP-10 | CCTGCTTTGTCCTTTGATTCAGT | CGGGATTCCAATGGGATCT | TCCTATTCTTTAAAGACAGGTACTTCTGGCGCA |
| MMP-11 | ATTGATGCTGCCTTCCAGGAT | GGGCGAGGAAAGCCTTCTAG | TCCTTCGTGGCCATCTCTACTGGAAGTTTG |
| MMP-12 | GAAACCCCCATCCTTGACAA | TTCCACCAGAAGAACCAGTCTTTAA | AGTCCACCATCAACTTTCTGTCACCAAAGC |
| MMP-13 | GGGCTCTGAATGGTTATGACATTC | AGCGCTCAGTCTCTTCACCTCTT | AAGGTTATCCCAGAAAAATATCTGACCTGGGATTC |
| MMP-14 | AGGAGACAGAGGTGATCATCATTG | GTCCCATGGCGTCTGAAGA | CCTGCCGGTACTACTGCTGCTCCTG |
| MMP-15 | ATCCCCTATGACCGCATTGAC | CCCCTGCCAGACACTGATG | ACACAGCATGGAGACCCTGGCTACCC |
| MMP-16 | GGCTACCTTCCACCGACTGA | CTTCATCCAGTCGATTGTGTTTCT | CTGCAGAGACCATGCAGTCAGCTCTAGCT |
| MMP-17 | GGCAGTATGTTCCTGCACTTCA | GCTAGCACTGCCCTCAGGAT | CCTGTGGACCTCAGTCTCTGCCAAGG |
| MMP-19 | GCCCATTTCCGGTCAGATG | AGGGATCCTCCAGACCACAAC | CCACAAGGGCCCGTATGAAGCAGC |
| MMP-20 | GATCAGGAGGATTAAGGAGCTACAAA | GGCGGTAGTTAGCCACATCAG | CCAGAATACAATGAATGTGATCAAGAAGCCTCG |
| MMP-21 | TCCAAAGAAGATGAGCCAAGTG | ACGCTGAATCGAGGTTTCTG | TTCCAGCAATAATGCCTCAAAACCACCC |
| MMP-23 | CAGACTGTTGACCATGTCGGTAA | GAAGGAAAGAACTCTGTATGTGAGGTT | CCGCTACACGCTGACACCGGC |
| MMP-24 | TATCATGGCTCCCTTCTACCAATAC | CTGCGGACCGGGAGTGT | CCAGCTGAGCCCTCTGGAGCCA |
| MMP-25 | TGGCTGTCTGGGCTACTGAA | GGTAGGCCCGAGCAAAGTG | AATTCTCAGTACCAGGAGCCTGACATCATTATCC |
| MMP-27 | AGGATAATAAAGTGCTTCCCAGGA | AAGAAATAGAGGAATCCATTATGTTGG | TCGCCTCCGTGTGGATGCTGTC |
| MMP-28 | CCACTTGGACAGAGAGGATCAGT | AAGCGTTTCTTACGCCTCATTT | CTGCTTGCTGGACACCGAGCCAA |
MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase.