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. 2015 Jan 18;56(2):294–304. doi: 10.1093/jrr/rru114

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© The Author 2015. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology.

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Fig. 6. — Visualization of enzyme activity and membrane integrity with FDA–PI double-staining. Cells were irradiated with X-ray or CI radiation and stained using FDA–PI, as described in Materials and Methods. A (X-ray) and B (CI) represent X-ray and CI radiation exposure, respectively. Left panel: fluorescence micrographs of incorporated FDA and PI. Right panel: phase contrast micrographs of the same cells. Scale is 20 μm.

Fig. 6. — Visualization of enzyme activity and membrane integrity with FDA–PI double-staining. Cells were irradiated with X-ray or CI radiation and stained using FDA–PI, as described in Materials and Methods. A (X-ray) and B (CI) represent X-ray and CI radiation exposure, respectively. Left panel: fluorescence micrographs of incorporated FDA and PI. Right panel: phase contrast micrographs of the same cells. Scale is 20 μm.