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Consecutive sections of radial arteries stained with alizarin red (a, c, and e) and immunostained for AGE (b, d, and e) showing calcifications of various grades 0 (a), 2 (c), and 4 (e). AGE deposits immunolabeled in red color presenting low (b and f) and high (d) intensities of the immunofluorescence in media. Bar = 200 μm.
